in silico identification of mirnas and their target genes and analysis of gene co-expression network in saffron (crocus sativus l.) stigma

نویسندگان

zahra zinati

agroecology department, college of agriculture and natural resources of darab, shiraz university, iran roohollah shamloo-dashtpagerdi

crop production and plant breeding department, college of agriculture, shiraz university, iran ali behpouri

agroecology department, college of agriculture and natural resources of darab, shiraz university, iran

چکیده

as an aromatic and colorful plant of substantive taste, saffron (crocus sativus l.) owes such properties of matter to growing class of the secondary metabolites derived from the carotenoids, apocarotenoids. regarding the critical role of micrornas in secondary metabolic synthesis and the limited number of identified mirnas in c. sativus, on the other hand, one may see the point how the characterization of mirnas along with the corresponding target genes in c. sativus might expand our perspectives on the roles of mirnas in carotenoid/apocarotenoid biosynthetic pathway. a computational analysis was used to identify mirnas and their targets using est (expressed sequence tag) library from mature saffron stigmas. then, a gene co-expression network was constructed to identify genes which are potentially involved in carotenoid/apocarotenoid biosynthetic pathways.  est analysis led to the identification of two putative mirnas (mir414 and mir837-5p) along with the corresponding stem-looped precursors. to our knowledge, this is the first report on mir414 and mir837-5p in c. sativus. co-expression network analysis indicated that mir414 and mir837-5p may play roles in c. sativus metabolic pathways and led to identification of candidate genes including six transcription factors and one protein kinase probably involved in carotenoid/apocarotenoid biosynthetic pathway. presence of transcription factors, mirnas and protein kinase in the network indicated multiple layers of regulation in saffron stigma. the candidate genes from this study may help unraveling regulatory networks underlying the carotenoid/apocarotenoid biosynthesis in saffron and designing metabolic engineering for enhanced secondary metabolites.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

In silico identification of miRNAs and their target genes and analysis of gene co-expression network in saffron (Crocus sativus L.) stigma

As an aromatic and colorful plant of substantive taste, saffron (Crocus sativus L.) owes such properties of matter to growing class of the secondary metabolites derived from the carotenoids, apocarotenoids. Regarding the critical role of microRNAs in secondary metabolic synthesis and the limited number of identified miRNAs in C. sativus, on the other hand, one may see the point how the characte...

متن کامل

In silico identification of miRNAs and their target genes and analysis of gene co-expression network in saffron (Crocus sativus L.) stigma

As an aromatic and colorful plant of substantive taste, saffron (Crocus sativus L.) owes such properties of matter to growing class of the secondary metabolites derived from the carotenoids, apocarotenoids. Regarding the critical role of microRNAs in secondary metabolic synthesis and the limited number of identified miRNAs in C. sativus, on the other hand, one may see the point how the characte...

متن کامل

Identification, Sequencing and Stability Evaluation of Eight Reference Genes in Saffron (Crocus sativus L.)

Saffron (Crocus sativus L.) is the most valuable and expensive spice in the world. The stigmas of saffron are the source of valuable apocarotenoids such as crocin, picrocrocin and safranal. transcriptomic and expression studies of genes are important steps in investigating of secondary metabolites in plants. One of the important prerequisites for such studies is the existence of reliable and st...

متن کامل

analysis of power in the network society

اندیشمندان و صاحب نظران علوم اجتماعی بر این باورند که مرحله تازه ای در تاریخ جوامع بشری اغاز شده است. ویژگیهای این جامعه نو را می توان پدیده هایی از جمله اقتصاد اطلاعاتی جهانی ، هندسه متغیر شبکه ای، فرهنگ مجاز واقعی ، توسعه حیرت انگیز فناوری های دیجیتال، خدمات پیوسته و نیز فشردگی زمان و مکان برشمرد. از سوی دیگر قدرت به عنوان موضوع اصلی علم سیاست جایگاه مهمی در روابط انسانی دارد، قدرت و بازتولید...

15 صفحه اول

Safety evaluation of saffron stigma (Crocus sativus L.) aqueous extract and crocin in patients with schizophrenia

OBJECTIVES Saffron is the stigma of Crocus sativus L., which has the potentials to play a role in the treatment of many diseases. Although many researches are now going on this precious spice, there are few data on saffron safety in human, especially in patients with chronic mental illnesses. This study aimed to evaluate the short-term safety and tolerability of both saffron and crocin (its maj...

متن کامل

the stady and analysis of rice agroclimatology in lenjan

the west of esfahan province, iran, is one of the most important agricultural areas throughout the country due to the climate variability and life-giving water of zayanderood river. rice is one of the major and economic crops in this area. the most important climatic elements in agricultural activities which should be considered include temperature, relative humidity, precipitation and wind. so...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
molecular biology research communications

جلد ۵، شماره ۴، صفحات ۲۳۳-۲۴۶

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023